People

Dr. Jens Appel
Dr. Jens Appel


Department Solar Materials
Helmholtz Centre for Environmental Research
Kubus, Room 409
04318 Leipzig
Germany
Tel.: +49-341-235 46 95
Fax.: +49-341-235 45 12 86

E-Mail:
 
 
 
 
 
 
 

 

Research Interests

  • photosynthetic electron transfer
  • biochemistry of cyanobacterial hydrogenases
  • biohydrogen production

CV / Scientific Career


Professional Career

  

since 09/2016                       

   

Scientist, Department of Solar Materials
Helmholtz Center for Environmental Research - UFZ, Leipzig
 

 2010 - 2016

 

Adjunct professor at Arizona State University, senior scientist at the Botanical Institute of the University of Kiel, and teacher at the Stiftung Louisenlund
 

2008 - 2009

  

Assistant Professor at Arizona State University
 

2006 - 2007


  

Leader of the project „Molecular ecology and monitoring of hydrogenases and nitrogenases in the bacterioplankton of the North Atlantic, the North Sea and the Baltic Sea “
 

1999 - 2005

  

Post doc in the group of Prof. Schulz at the Botanical Institute at the
University of Kiel
 

1995 - 1999

 

  

Ph. D. in the group of Prof. Senger at the University of Marburg ("Function of the bidirectional hydrogenase and the NAD(P)H: plastoquinone oxidoreductase (complex I) in Synechocystis sp. PCC 6803")

Publications
Peer Reviewed Articles
2016
  • Chen X., Schreiber K. , Appel J., Makowka A., Fähnrich B., Roettger M., Hajirezaei M., Sönnichsen F., Schönheit P., Martin W.F. and Gutekunst K. (2016)
    The Entner-Doudoroff pathway is an overlooked glycolytic route in cyanobacteria and plants.
    Proc Natl Acad Sci U S A - PNAS, 113(19):5441-5446, DOI-Link
2014
  • Beimgraben C., Gutekunst K., Opitz F. and Appel J. (2014)
    HypD as a marker for NiFehydrogenases in microbial communities of surface waters.
    Applied and Environmental Microbiology, 80:3776-3782, DOI-Link
  • Burroughs NJ, Boehm M., Eckert C., Spence EM, Yu J., Nixon P.J., Appel J., Mullineaux C.W. and Bryan S.J. (2014)
    Solar powered biohydrogen production requires specific localization of the hydrogenase Energy .
    Env. Sci., 7:3791-3800
  • Gutekunst K., Chen X., Schreiber K. , Kaspar U., Makam S. and Appel J. (2014)
    The bidirectional NiFe-hydrogenase in Synechocystis sp. PCC 6803 is reduced by flavodoxin and ferredoxin and is essential under mixotrophic, nitrate-limiting conditions.
    Journal of Biological Chemistry, 289:1930-1937
2011
  • Bernát G., Appel J., Ogawa T. and Rögner M. (2011)
    Distinct Roles of Multiple NDH-1 Complexes in the Cyanobacterial Electron Transport Network as Revealed by Kinetic Analysis of P700+ Reduction in Various ndh-deficient Strains of Synechocystis sp. PCC6803 .
    J Bacteriol, 193:292-295
  • Inoue S., Ejima K., Iwai E., Hayashi H., Appel J., Tyystjärvi E., Murata N. and Nishiyama Y. (2011)
    Protection by α-tocopherol of the repair of photosystem II during photoinhibition in Synechocystis sp. PCC 6803.
    Biochim Biophys Acta, 1807:234-241
  • McIntosh C.L., Germer F., Schulz R., Appel J. and Jones A.K. (2011)
    The NiFe-hydrogenase of the cyanobacterium Synechocystis sp. PCC 6803 is working bidirectional with a bias to H2 production.
    J Am Chem Soc, 133:11308-11319
  • Appel J. (2011)
    The physiology and functional genomics of cyanobacterial hydrogenases and approaches towards biohydrogen production.
    Functional Genomics and Evolution of Photosynthetic Systems Eds. RL Burnap and WFJ Vermaas, 33:357-381
2010
  • Barz M., Beimgraben C., Staller T., Germer F., Opitz F., Marquardt C. , Schwarz C., Gutekunst K., Vanselow K.H., Schmitz R., LaRoche J., Schulz R. and Appel J. (2010)
    Distribution analysis of hydrogenases in surface waters of marine and freshwater environments.
    PlosOne, (e13846)
  • Schwarz C., Poss Z. , Hoffmann D. and Appel J. (2010)
    Hydrogenases and hydrogen metabolism in photosynthetic prokaryotes.
    Adv Exp Med Biol, 675:305-348
2009
  • Schultze M., Forberich B., Rexroth S., Dyczmons N.G., Rögner M. and Appel J. (2009)
    Localization of cytochrome b6f complexes implies an incomplete respiraotry chain in cytoplasmic membranes of the cyanobacterium Synechocystis p. PCC 6803.
    Biochim Biophys Acta-Bionergetics, 1787:1479-1485
  • Germer F., Zebger I., Saggu M., Lendzian F., Schulz R. and Appel J. (2009)
    Overexpression, isolation, and spectroscopic characterization of the bidirectional [NiFe] hydrogenase from Synechocystis sp.
    PCC 6803    .
    J Biol Chem, 284:36462-36472
2007
  • Gutthann F., Egert M., Marques A. and Appel J. (2007)
    Inhibition of respiration and nitrate assimilation enhances hydrogen evolution in Synechocystis sp. PCC 6803.
    Biochem. Biophys. Acta- Bioenergetics, 1767:161-169
2006
  • Gutekunst K., Hoffmann D., Lommer M., Egert M., Suzuki I., Schulz-Friedrich R. and Appel J. (2006)
    Metal dependence and intracellalar regulation of the bidirectional NiFe-hydrogenase in Synechocystis sp. PCC 6803.
    International Journal of Hydrogen Energy, 31:1452-1459
  • Hoffmann D., Gutekunst K., Klissenbauer M., Schulz-Friedrich R. and Appel J. (2006)
    Mutagenesis of all hydrogenase accessory genes of Synechocystis sp. PCC 6803 –  additional homologs of hyp A and hyp B are not active in hydrogenase maturation.
    FEBS Journcal, 273:4516-4527
  • Ludwig M., Schulz-Friedrich R. and Appel J. (2006)
    Occurrence of hydrogenases in cyanobacteria and anoxygenic photosynthetic bacteria: implications for the phylogenetic origin of cyanobacterial and algal hydrogenases.
    J Mol Evol, 63:758-768
2005
  • Backasch N., Schulz-Friedrich R. and Appel J. (2005)
    Influences on tocopherol biosynthesis in the cyanobacterium Synechocystis sp. PCC 6803.
    J Plant Phys, 162:758-766
  • Gutekunst K., Phunpruch S., Schwarz C., Schuchardt S., Schulz-Friedrich R. and Appel J. (2005)
    LexA regulates the bidirectional hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803 as a transcription activator.
    Molecular Microbiology, 58:810-823
2004
  • Zhang P., Battchikova N., Jansen T., Appel J., Ogawa T. and Aro E.M. (2004)
    Expression and functional roles of the two distinct NDH-1 complexes and the carbon acquisition complex NdhD3/NdhF3 /CupA/Sll1735 in Synechocystis sp. PCC 6803.
    Plant Cell, 16:3326-3340
2002
  • Dähnhardt D., Falk J., Appel J., van der Kooij T.A.W., Schulz-Friedrich R. and Krupinska K. (2002)
    The hydroxyphenylpyruvate dioxygenase from Synechocystis sp. PCC 6803 is not required for
    plastoquinone biosynthesis    .
    FEBS Lett, (523):177-181
2000
  • Appel J., Phunpruch S., Steinmüller K. and Schulz R. (2000)
    The bidirectional hydrogenase of Synechocystis sp. PCC 6803 works as an electron valve during photosynthesis.
    Arch Microbiol, 173:333-338
1999
  • Kösling S., Appel J., Schulz R. and Steinmüller K. (1999)
    The complex I-homologous NAD(P)Hplastoquinone-oxidoreductase of Synechocystis sp. PCC 6803.
    The phototrophic prokaryotes, ed. Peschek GA, Löffelhardt W, Schmetterer G, Kluwer Academic Publishers, :211-215
1998
  • Appel J. and Schulz R. (1998)
    Hydrogen metabolism in organisms with oxygenic photosynthesis - hydrogenases as important regulatory devices for a proper redox poising?
    J Photochem Photobiol B: Biol, 47:1-11
1996
  • Appel J. and Schulz R. (1996)
    Sequence analysis of an operon of a NAD(P)-reducing nickel hydrogenase from the cyanobacterium Synechocystis sp. PCC 6803 gives additional evidence for direct coupling of the enzyme to NAD(P)H-dehydrogenase (complex I).
    Biochim Biophys Acta, 1298:141-147