People

Prof. Dr. Bruno Bühler
Prof. Dr. Bruno Bühler


Department of Solar Materials
Helmholtz Centre for Environmental Research
Kubus, Room 410
04318 Leipzig
Germany
Tel.: +49-341-235 46 87
Fax.: +49-341-235 45 12 86

E-Mail:
 
 
 
 
 
 
 

 

Research Interests

Photobiotechnology, (continuous) redox and multistep biocatalysis using microbial cell factories, highly productive and specific oxyfunctionalizations, metabolic engineering – heterologous pathway design and systems biotechnology in Cyanobacteria, Escherichia coli, Pseudomonads, and Corynebacterium glutamicum, reaction engineering, sustainability


CV / Scientific Career

Education

  01/2014                  

 
 

Habilitation, TU Dortmund University, Venia Legendi in Biotechnology and
Technical Microbiology

  1998 - 2003




  PhD studies, Dr. sc. nat. ETH Swiss Federal Institute of Technology (ETH) Zurich,
Thesis: “Specific Oxyfunctionalization of Pseudocumene by Multistep Biocatalysis - Mechanism
and Scale Up. 'Supervision: Prof. Dr. A. Schmid, Prof. Dr. B. Witholt
Awarded with the ETH medal for outstanding PhD theses

 

1998



 

Diploma, Dipl. Natw. ETH Swiss Federal Institute of Technology (ETH) Zurich
Thesis: “Analysis of the mRNA Pattern of the pha Locus of Pseudomonas
oleovorans
and Evaluation of Possible Functions of the PhaF Protein”
Supervision: Dr. M. A. Prieto, Dr. B. Kessler, Prof. Dr. B. Witholt

  1993 - 1998

  Study of Biology/ Biochemistry, specialization in Biotechnology, Swiss Federal Institute
of Technology (ETH) Zurich, Switzerland

Positions

 

10/2015 - present


   

Professor for Applied Biocatalysis
Faculty I of Natural Science – Biological Science, Martin Luther University Halle-Wittenberg,
Halle, Germany

 

07/2015 -  present


 

Group Leader
Head of “Applied Biocatalysis Group” in the Department Solar Materials, Helmholtz
Centre for Environmental Research - UFZ, Leipzig, Germany

 

07/2015 - 12/2016


 

Visiting Scientist
Laboratory of Chemical Biotechnology, Department of Biochemical &
Chemical Engineering, TU Dortmund University, Dortmund, Germany

 

10/2014 - 06/2015


 

Visiting Scientist
Department Solar Materials, Helmholtz Centre for Environmental
Research - UFZ, Leipzig, Germany

  11/2004 - 06/2015



  Group Leader
Head of “Applied Biocatalysis Group” in the Laboratory of Chemical
Biotechnology, Department of Biochemical & Chemical Engineering,
TU Dortmund University, Dortmund, Germany
  07/2003 - 10/2004

  Post Doctoral Fellow
Institute of Biotechnology, ETH Zurich, Switzerland
   06/1998 - 06/2003

  Research Scientist
Institute of Biotechnology, ETH Zurich, Switzerland
Publications
Peer Reviewed Articles
2017
  • Hoschek A, Bühler B. and Schmid A. (2017)
    Overcoming the gas-liquid mass transfer of O2 by means of photosynthetic water oxidation coupled with biocatalytic oxyfunctionalization.
    Angewandte Chemie International Edition, Accepted
  • Kadisch M., Schmid A. and Bühler B. (2017)
    Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing.
    Journal of Industrial Microbiology & Biotechnology, 44(3):339–351, DOI-Link
  • Theodosiou E., Breisch M., Julsing M. K., Falcioni F., Bühler B. and Schmid A. (2017)
    An artificial TCA cycle selects for efficient α-ketoglutarate dependent hydroxylase catalysis in engineered Escherichia coli.
    Biotechnology and Bioengineering, 114(7):1511-1520, DOI-Link
  • Kadisch M., Julsing M. K., Schrewe M., Jehmlich N., Scheer B., von Bergen M., Schmid A. and Bühler B. (2017)
    Maximization of cell viability rather than biocatalyst activity improves whole-cell ω-oxyfunctionalization performance.
    Biotechnology and Bioengineering, 114(4):874-884, DOI-Link
  • Kadisch M.*, Willrodt C.*, Hillen M, Bühler B. and Schmid A. (2017)
    Maximizing the stability of metabolic engineering-derived whole-cell biocatalysts.
    Biotechnology Journal, 12(8):1600170, DOI-Link
    * These authors contributed equally to this work.
  • Volmer J., Schmid A. and Bühler B. (2017)
    The application of constitutively solvent-tolerant P. taiwanensis VLB120ΔCΔttgV for stereospecific epoxidation of toxic styrene alleviates carrier solvent use.
    Biotechnology Journal, 12:1600558, DOI-Link
2016
  • Willrodt C., Hoschek A, Bühler B., Schmid A. and Julsing M. K. (2016)
    Decoupling production from growth by magnesium sulfate limitation boosts de novo limonene production.
    Biotechnology and Bioengineering, 113(6):1305-1314, DOI-Link
  • Ladkau N., Aßmann M., Schrewe M., Julsing M. K., Schmid A. and Bühler B. (2016)
    Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli.
    Metabolic Engineering, 36:1-9, DOI-Link
2015
  • Willrodt C., Hoschek A, Bühler B., Schmid A. and Julsing M. K. (2015)
    Coupling limonene formation and oxyfunctionalization by mixed-culture resting cell fermentation.
    Biotechnology & Bioengineering, 112(9):1738-1750, DOI-Link
  • Looße C., Galozzi S., Debor L., Julsing M. K., Bühler B., Schmid A., Barkovits K., Müller T. and Marcus K. (2015)
    Direct infusion-SIM as fast and robust method for absolute protein quantification in complex samples.
    EuPA Open Proteomics, 7:20-26, DOI-Link
  • Falcioni F., Bühler B. and Schmid A. (2015)
    Efficient hydroxyproline production from glucose in minimal media by Corynebacterium glutamicum.
    Biotechnology and Bioengineering, 112(2):322-330, DOI-Link
  • Volmer J., Schmid A. and Bühler B. (2015)
    Guiding bioprocess design by microbial ecology.
    Current Opinion in Microbiology, 25:25-32, DOI-Link
  • Lindmeyer M., Meyer D., Kuhn D., Bühler B. and Schmid A. (2015)
    Making variability less variable: matching expression system and host for oxygenase-based biotransformations.
    Journal of Industrial Microbiology and Biotechnology, 42(6):851-866, DOI-Link
  • Theodosiou E., Frick O., Bühler B. and Schmid A. (2015)
    Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation.
    Microbial Cell Factories, 14:108, DOI-Link
  • Brandenbusch C., Collins J., Glonke S., Hoffrogge R., Grunwald K., Bühler B., Schmid A. and Sadowski G. (2015)
    Process boundaries of irreversible scCO2-assisted phase separation in biphasic whole-cell biocatalysis.
    Biotechnology and Bioengineering, 112(11):2316-2323, DOI-Link
  • Collins J., Grund M., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2015)
    The dynamic influence of cells on the formation of stable emulsions in organic-aqueous biotransformations.
    Journal of Industrial Microbiology and Biotechnology, 42(7):1011-1026, DOI-Link
  • Lindmeyer M., Jahn M., Vorpahl C., Müller S., Schmid A. and Bühler B. (2015)
    Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains.
    Frontiers in Microbiology, 6:1042, DOI-Link
2014
  • Jahn M., Vorpahl C., Türkowsky D., Lindmeyer M., Bühler B., Harms H. and Müller S. (2014)
    Accurate determination of plasmid copy number on the single cell level using droplet digital PCR.
    Analytical Chemistry, 86(12):5969–5976, DOI-Link
  • Volmer J., Neumann C., Bühler B. and Schmid A. (2014)
    Engineering of Pseudomonas taiwanensis VLB120 for constitutive solvent tolerance and increased specific styrene epoxidation activity.
    Applied and Environmental Microbiology, 80(20):6539-6548, DOI-Link
  • Willrodt C., David C., Cornelissen S., Bühler B., Julsing M. K. and Schmid A. (2014)
    Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media.
    Biotechnology Journal, 9(8):1000–1012, DOI-Link
  • Schrewe M., Julsing M. K., Lange K., Czarnotta E, Schmid A. and Bühler B. (2014)
    Reaction and catalyst engineering to exploit kinetically controlled whole-cell multistep biocatalysis for terminal FAME oxyfunctionalization.
    Biotechnology and Bioengineering, 111(9):1820-1830, DOI-Link
  • Ladkau N., Schmid A. and Bühler B. (2014)
    The microbial cell - functional unit for energy-dependent multistep biocatalysis.
    Current Opinion in Biotechnology, 30:178-189, DOI-Link
2013
  • Steen A., Ütkür F.Ö., Borrero-de Acuna J. M., Bunk B., Roselius L., Bühler B., Jahn D. and Schobert M. (2013)
    Construction and characterization of nitrate and nitrite respiring Pseudomonas putida KT2440 strains for anoxic biotechnical applications.
    Journal of Biotechnology, 163(2):155-165, DOI-Link
  • Schrewe M., Ladkau N., Bühler B. and Schmid A. (2013)
    Direct terminal alkylamino-functionalization via multistep biocatalysis in one recombinant whole-cell catalyst.
    Advanced Synthesis and Catalysis, 355(9):1693-1697, DOI-Link
  • Falcioni F., Blank L. M., Frick O., Karau A., Bühler B. and Schmid A. (2013)
    Proline availability regulates proline-4-hydroxylase synthesis and substrate uptake in proline-hydroxylating recombinant Escherichia coli.
    Applied and Environmental Microbiology, 79(9):3091-3100, DOI-Link
  • Jahn M., Seifert J., von Bergen M., Schmid A., Bühler B. and Müller S. (2013)
    Subpopulation-proteomics in prokaryotic populations.
    Current Opinion in Biotechnology, 24(1):79-87, DOI-Link
  • Kuhn D., Fritzsch F. S. O., Zhang X., Wendisch V. F., Blank L. M., Bühler B. and Schmid A. (2013)
    Subtoxic product levels limit the epoxidation capacity of recombinant E. coli by increasing microbial energy demands.
    Journal of Biotechnology, 163(2):194-203, DOI-Link, PubMed
  • Schrewe M.*, Julsing M. K.*, Bühler B. and Schmid A. (2013)
    Whole-cell biocatalysis for selective and productive C-O functional group introduction and modification.
    Chemical Society Reviews, 42(15):6346 - 6377, DOI-Link
    * These authors contributed equally to this work.
  • Cornelissen S., Julsing M. K., Volmer J., Riechert O., Schmid A. and Bühler B. (2013)
    Whole-cell-based CYP153A6-catalyzed (S)-limonene hydroxylation efficiency depends on host background and profits from monoterpene uptake via AlkL.
    Biotechnology and Bioengineering, 110(5):1282-1292, DOI-Link
2012
  • Cornelissen S., Julsing M. K., Schmid A. and Bühler B. (2012)
    Comparison of microbial hosts and expression systems for mammalian CYP1A1 catalysis.
    Journal of Industrial Microbiology and Biotechnology, 39(2):275-287, DOI-Link
  • Ütkür F.Ö., Tran T.-T., Collins J., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2012)
    Integrating organic-aqueous biocatalysis and product recovery using quinaldine 4-oxidase in living P. putida KT2440 (pKP1).
    Journal of Industrial Microbiology & Biotechnology, 39(7):1049-1059, DOI-Link
  • Kuhn D., Bühler B. and Schmid A. (2012)
    Production host selection for asymmetric styrene epoxidation: Escherichia coli vs. solvent tolerant Pseudomonas.
    Journal of Industrial Microbiology and Biotechnology, 39(8):1125-1133, DOI-Link
  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2012)
    Resting cells of recombinant E. coli show high epoxidation yields on energy source and high sensitivity to product inhibition.
    Biotechnology and Bioengineering, 109(5):1109-1119, DOI-Link
  • Braun A., Geier M., Bühler B., Schmid A., Mauersberger S. and Glieder A. (2012)
    Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes.
    Microbial Cell Factories, 11:106, DOI-Link
  • Kuhn D., Julsing M. K., Heinzle E. and Bühler B. (2012)
    Systematic optimization of a biocatalytic two-liquid phase oxyfunctionalization process guided by ecological andeconomic assessment.
    Green Chemistry, 14(3):645 - 653, DOI-Link
  • Julsing M. K.*, Schrewe M.*, Cornelissen S., Hermann I., Schmid A. and Bühler B. (2012)
    The outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in E. coli.
    Applied and Environmental Microbiology, 78(16):5724-5733, DOI-Link
    * These authors contributed equally to this work.
  • Escapa I. F., Garcia J. L., Bühler B., Blank L. M. and Prieto M.A. (2012)
    The polyhydroxyalkanoate metabolism controls carbon and energy spillage in Pseudomonas putida.
    Environmental Microbiology, 14(4):1049-1063, DOI-Link
2011
  • Cornelissen S., Liu S, Deshmukh A.T., Schmid A. and Bühler B. (2011)
    Cell physiology can determine the efficiency of cytochrome P450-catalyzed C-H-oxyfunctionalization.
    Journal of Industrial Microbiology and Biotechnology, 38:1359-1370, DOI-Link
  • Ladkau N., Hermann I., Bühler B. and Schmid A. (2011)
    Enzyme catalyzed laurolactam synthesis via intramolecular amide bond formation in aqueous solution.
    Advanced Synthesis and Catalysis, 353(13):2501-2510, DOI-Link
  • Hollmann F., Arends I. W. C. E, Bühler K., Schallmey A. and Bühler B. (2011)
    Enzyme-mediated oxidations for the chemist.
    Green Chemistry, 13:226-265, DOI-Link
  • Schrewe M., Magnusson A., Willrodt C., Bühler B. and Schmid A. (2011)
    Kinetic analysis of terminal and unactivated C-H bond oxyfunctionalization in fatty acid methyl esters by monooxygenase-based whole-cell biocatalysis.
    Advanced Synthesis and Catalysis, 353:3485 – 3495, DOI-Link
  • Ütkür F.Ö., Gaykawad S., Bühler B. and Schmid A. (2011)
    Regioselective aromatic hydroxylation of quinaldine with water using quinaldine 4-oxidase in recombinant Pseudomonas putida.
    Journal of Industrial Microbiology and Biotechnology, 38(8):1067-1077, DOI-Link
2010
  • Brandenbusch C., Bühler B., Hoffmann P., Sadowski G. and Schmid A. (2010)
    Efficient phase separation and product recovery in organic-aqueous bioprocessing using supercritical carbon dioxide.
    Biotechnology and Bioengineering, 107(4):642-651, DOI-Link
  • Kuhn D., Kholiq A., Heinzle E., Bühler B. and Schmid A. (2010)
    Intensification and economic and ecological assessment of a biocatalytic oxyfunctionalization process.
    Green Chemistry, 12(5):815-827, DOI-Link
  • Blank L. M., Ebert B. E., Bühler K. and Bühler B. (2010)
    Redox biocatalysis and metabolism: molecular mechanisms and metabolic network analysis.
    Antioxidants & Redox Signaling, 13(3):349-394, DOI-Link, PubMed
  • Kuhn D., Blank L. M., Schmid A. and Bühler B. (2010)
    Systems biotechnology - rational biocatalyst and bioprocess design.
    Engineering in Life Sciences, 10(5):384–397, DOI-Link
2008
  • Julsing M. K., Cornelissen S., Bühler B. and Schmid A. (2008)
    Heme-iron oxygenases: powerful industrial biocatalysts?
    Current Opinion in Chemical Biology, 12(2):177-186, DOI-Link, PubMed
  • Blank L. M., Ebert B. E., Bühler B. and Schmid A. (2008)
    Metabolic capacity estimation of Escherichia coli as platform for redox biocatalysis: Constraint based modeling and experimental verification.
    Biotechnology and Bioengineering, 100(6):1050-1065, DOI-Link, PubMed
  • Blank L. M., Ionidis G., Ebert B. E., Bühler B. and Schmid A. (2008)
    Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase.
    FEBS Journal, 275(20):5173-5190, DOI-Link, PubMed
  • Bühler B., Park J. B., Blank L. M. and Schmid A. (2008)
    NADH availability limits asymmetric biocatalytic epoxidation in growing recombinant Escherichia coli.
    Applied and Environmental Microbiology, 74(5):1436-1446, DOI-Link, PubMed
2007
  • Park J. B., Bühler B., Panke S., Witholt B. and Schmid A. (2007)
    Carbon metabolism and product inhibition determine the epoxidation efficiency of solvent tolerant Pseudomonas sp. strain VLB120ΔC.
    Biotechnology and Bioengineering, 98(6):1219-1229, DOI-Link, PubMed
2006
  • Bühler B., Straathof A. J. J., Witholt B. and Schmid A. (2006)
    Analysis of two-liquid-phase multistep biooxidation based on a process model: Indications for biological energy shortage.
    Organic Process Research and Development, 10(3):628-643, DOI-Link
  • Meyer D., Bühler B. and Schmid A. (2006)
    Process and catalyst design objectives for specific redox biocatalysis.
    Advances in Applied Microbiology, 59:53-91, DOI-Link, PubMed
  • Park J. B.*, Bühler B.*, Habicher T., Hauer B., Panke S., Witholt B. and Schmid A. (2006)
    The efficiency of recombinant Escherichia coli as biocatalyst for stereospecific epoxidation.
    Biotechnology and Bioengineering, 95(3):501-512, DOI-Link, PubMed
    * These authors contributed equally to this work.
2004
  • Bühler B. and Schmid A. (2004)
    Process implementation aspects for biocatalytic hydrocarbon oxyfunctionalisation.
    Journal of Biotechnology, 113(1-3):183-210, DOI-Link, PubMed
2003
  • Bühler B., Bollhalder I., Hauer B., Witholt B. and Schmid A. (2003)
    Chemical biotechnology for the specific oxyfunctionalization of hydrocarbons an a technical scale.
    Biotechnology and Bioengineering, 82(7):833-842, DOI-Link, PubMed
  • Bühler B., Bollhalder I., Hauer B., Witholt B. and Schmid A. (2003)
    Use of the two-liquid phase concept to exploit kinetically controlled multistep biocatalysis.
    Biotechnology and Bioengineering, 81(6):683-694, DOI-Link, PubMed
2002
  • Bühler B., Witholt B., Hauer B. and Schmid A. (2002)
    Characterization and application of xylene monooxygenase for multistep biocatalysis.
    Applied and Environmental Microbiology, 68(2):560-568, DOI-Link, PubMed
  • Schmid A., Hollmann F., Park J. B. and Bühler B. (2002)
    The use of enzymes in the chemical industry in Europe.
    Current Opinion in Biotechnology, 13(4):359-366, DOI-Link, PubMed
2000
  • Bühler B., Schmid A., Hauer B. and Witholt B. (2000)
    Xylene monooxygenase catalizes the multistep oxygenation of toluene and pseudocumene to corresponding alcohols, aldehydes, and acids in Escherichia coli JM101.
    Journal of Biological Chemistry, 275(14):10085-100092, PubMed
1999
  • Prieto M.A., Bühler B., Jung K., Witholt B. and Kessler B. (1999)
    PhaF, a polyhydroxyalkanoate-granule-associated protein of Pseudomonas oleovorans GPo1 involved in the regulatory expression system of pha genes.
    Journal of Bacteriology, 181(3):858-868, PubMed
Books & Bookchapters
2012
  • Hollmann F., Bühler K. and Bühler B. (2012)
    Oxidation of Alcohols, Aldehydes, and Acids.
    in Drauz, K., Gröger, H., May, O. Enzyme Catalysis in Organic Synthesis.
    Wiley-VCH, Weinheim, Germany, Vol. 3:1325-1437, ISBN: 978-3-527-32547-4
  • Bühler B., Bühler K. and Hollmann F. (2012)
    Oxyfunctionalization of C-C-Multiple Bonds.
    in Drauz, K., Gröger, H., May, O. Enzyme Catalysis in Organic Synthesis.
    Wiley-VCH, Weinheim, Germany, Vol. 3:1269-1324, ISBN: 978-3-527-32547-4
  • Ütkür F.Ö., Collins J., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2012)
    Regioselective Aromatic Hydroxylation of Quinaldine Using Living Pseudomonas putida Cells Containing Quinaldine 4-oxidase.
    in John Whitthal and Peter Sutton Practical Methods in Biocatalysis and Biotransformations 2.
    John Wiley & Sons Ltd, Chichester, UK:153-157, ISBN: 978-1-119-99139-7
2011
  • Brandenbusch C., Bühler B., Sadowski G. and Schmid A. (2011)
    Biokatalytische Epoxidierung von Styrol zu enantiomerenreinem (S)-Styroloxid.
    in Chmiel H. Bioprozesstechnik.
    Spektrum Akademischer Verlag, Heidelberg, Germany:486-488, ISBN: 978-3-8274-2476-1, Amazon
2009
  • Bühler B., Blank L. M., Ebert B. E., Bühler K. and Schmid A. (2009)
    Energy and cofactor issues in fermentation and oxyfunctionalisation processes.
    in Smolke C. D. The metabolic pathway engineering handbook.
    CRC Press, Boca Raton, Florida, USA, Vol. 2:21 1-32, ISBN: 978-1-4200-7765-0
2008
  • Eissen M., Geisler G., Bühler B., Fischer C., Hungerbuehler K., Schmid A. and Carreira E. M. (2008)
    Mass Balances and Life Cycle Assessment.
    in Lapkin A. and Constable D. Green Chemistry Metrics: Measuring and Monitoring Sustainable Processes.
    John Wiley & Sons Ltd, Chichester, West Sussex, UK:200-227
2005
  • Bühler B. and Schmid A. (2005)
    Selective enzymatic hydroxylations.
    in Dyker G. Handbook of C-H transformations.
    Wiley-VCH, Weinheim, Germany, Vol. 2:516-529
2003
  • Bühler B. (2003)
    PhD Thesis: Specific oxyfunctionalization of pseudocumene by multistep biocatalysis - mechanism and scale up.
    Swiss Federal Institute of Technology (ETH), Zurich, Switzerland, Direct-Link
2002
  • Hollmann F., Bühler B. and Schmid A. (2002)
    Oxidation of alcohols, phenols, aldehydes, acids and C-N bonds.
    in Drauz K. and Waldmann H. Enzyme catalysis in organic synthesis.
    Wiley-VCH, Weinheim, Germany, Vol. III:1108-1202, 1245-1261, ISBN: 3-527-29949-1
Patent applications
2017
  • Schmid A., Hoschek A and Bühler B. (2017)
    Verfahren zur bioreaktiven Extraktion erzeugten Sauerstoffs aus einem Reaktionsraum, sowie Verwendung von phototrophen Mikroorganismen bei der Gewinnung von Wasserstoff.
    Germany, 2017030615575400DE, filed
2013
  • Sadowski G., Brandenbusch C., Collins J. and Bühler B. (2013)
    Applied catastrophic phase inversion.
    Europe, EP 13 005 234.3, priority: 6.11.2013, filed
2011
  • Schmid A., Bühler B., Ladkau N. and Hermann I. (2011)
    Method for omega-aminocarboxylic acid condensation.
    Europe, WO/2012/156151 / PCT/EP2012/056251 / priority: 11004029.2 16.05.2011 EP
2010
  • Pötter M., Schmid A., Bühler B., Hennemann H.-G., Julsing M. K., Schaffer S., Haas T., Schrewe M., Cornelissen S., Roos M. and Häger H. (2010)
    Biocatalytic oxidation process with alkL-gene product.
    WO 2011/131420 / PCT/EP2011/053834 / priority: 10 2010 015 807.0 20.04.2010 DE
2007
  • Sadowski G., Schmid A., Brandenbusch C., Bühler B. and Görnert M. (2007)
    Method for processing coalescence-inhibited emulsions from whole-cell biotransformations with compressed or supercritical gases.
    WO/2009/012754 / EP2170481 / DE 10 2007 034 258.8 / US 8,431,358 B2
  • Karau A., Sieber V., Haas T., Häger H., Grammann K., Bühler B., Blank L. M. and Schmid A. (2007)
    omega-amino carboxylic acids, omega-amino carboxylic acid esters, or recombinant cells which produce lactams thereof.
    WO/2009/077461 / PCT/EP2008/067447 / priority: 102007060705.0 17.12.2007 DE
2001
  • Schmid A., Hauer B., Bühler B. and Witholt B. (2001)
    Method for the oxidation of aromatic compunds.
    WO/2002/081718 / PCT/EP2002/003803 / priority: 101 17 359.8 06.04.2001 DE
1999
  • Schmid A., Bühler B., Hauer B. and Witholt B. (1999)
    Microbiological method for producing aromatic aldehydes and/or carboxylic acids.
    WO/2001/031047 / PCT/EP2000/010552 / priority: 199 51 768.1 27.10.1999 DE
Oral Presentations
2016
  • Bühler B. (2016)
    Productive two-liquid phase whole-cell biocatalysis on the verge of industrial implementation.
    Seminar at the Department of Biotechnology at the TU Delft, 7th of January 2016, Delft, The Netherlands
  • Bühler B. (2016)
    Integrated bioprocess development for whole-cell biocatalysis .
    Fields of Expertise meeting, TU Graz, 3rd of November 2016, Graz, Austria
  • Bühler B. (2016)
    Productive whole-cell oxygenase and cascade biocatalysis.
    2016 KSBB Spring Meeting and International Symposium, 21st - 22nd of April 2016, Gyeongju, South Korea
  • Bühler B. and Maskow T. (2016)
    Biotechnology for the chemical industry.
    UFZ EnergyDays 2016, 22nd - 23rd of March 2016, Leipzig, Germany
  • Bühler B. (2016)
    Whole-cell biocatalysis - status and future prospects.
    Industrial Biocatalysis; 8th Wädenswil Day of Life Science hosts CCBIO Symposium, 20th of June 2016, Wädenswil, Switzerland
  • Bühler B., Schrewe M., Ladkau N., Falcioni F., Julsing M. K. and Schmid A. (2016)
    The microbial cell – functional unit for multistep biocatalysis.
    Seminar at Ewha Womans University, 19th of April 2016, Seoul, South Korea
  • Bühler B. (2016)
    Productive whole-cell biocatalysis.
    Conference on Green Chemistry and White Biotechnology, 12th - 13th of May 2016, Gembloux, Belgium
  • Bühler B., Volmer J., Lindmeyer M. and Schmid A. (2016)
    How to profit from the promising properties of Pseudomonads for productive whole-cell biocatalysis.
    European Symposium on Biochemical Engineering Sciences (ESBES) 2016, 11th -14th of September 2016, Dublin, Ireland
2015
  • Bühler B., Volmer J., Lindmeyer M. and Schmid A. (2015)
    Exploiting and engineering solvent tolerance of Pseudomonads for whole-cell biocatalysis.
    Sino-­German Symposium on Biobased Chemicals and Biorefinery, 8. - 9. October 2015, Frankfurt, Germany
  • Bühler B. (2015)
    Productive microbial redox and multistep biocatalysis utilizing renewable resources.
    Workshop: Biotechnologie der stofflichen und energetischen Nutzung erneuerbarer Ressourcen, 1st - 2nd of December , UFZ Leipzig, Germany
2014
  • Bühler B. (2014)
    Biocatalyst and reaction engineering for productive whole-cell biocatalysis .
    Sino-German Symposium on Biobased Chemicals and Biorefinery, 7. - 9. October 2014, Beijing, China
  • Bühler B., Schrewe M., Ladkau N., Falcioni F., Julsing M. K. and Schmid A. (2014)
    The microbial cell - functional unit for multistep biocatalysis.
    3rd Multistep Enzyme Catalyzed Processes Congress (MECP14), 7. - 10. April 2014, Madrid, Spain
2013
  • Bühler B., Schrewe M., Julsing M. K., Ladkau N., Cornelissen S., Hillen M, Falcioni F., Kuhn D., Volmer J., Lindmeyer M., Blank L. M. and Schmid A. (2013)
    Orthologous reaction and pathway engineering enabling productive whole-cell biocatalysis.
    Seminar of the Institute of Applied Synthetic Chemistry, TU Vienna, 24. October 2013, Vienna, Austria
  • Bühler B. (2013)
    Biotechnologie für die Chemieindustrie - Biocatalyst and Reaction Engineering for Productive Multistep and Redox Biocatalysis .
    Tag des BCI, TU Dortmund, 11. October 2013, Dortmund, Germany
  • Bühler B., Schrewe M., Julsing M. K., Ladkau N., Cornelissen S., Hillen M, Falcioni F., Kuhn D., Volmer J., Blank L. M. and Schmid A. (2013)
    Biocatalyst and Reaction Engineering for Productive Multistep and Redox Biocatalysis .
    Biotrans 2013, 21. - 25. July 2013, Manchester, UK
  • Bühler B., Falcioni F., Ladkau N., Theodosiou E., Schrewe M., Fu J., Bühler K., Julsing M. K. and Schmid A. (2013)
    Engineering of microbes for oxyfunctionalization and multistep biocatalysis based on renewable resources.
    Bio-Alternatives 2013, 3. - 4. April 2013, Graz, Austria
2012
  • Bühler B., Ladkau N., Schrewe M., Julsing M. K. and Schmid A. (2012)
    Terminal oxy- and aminofunctionalization of fatty acid methyl esters via heterologous pathway engineering.
    ESBES - 9th European Symposium on Biochemical Engineering Science, 23. - 26. September 2012, Istanbul, Turkey
  • Bühler B., Kuhn D., Schrewe M., Julsing M. K., Ladkau N., Ütkür F.Ö., Cornelissen S., Blank L. M. and Schmid A. (2012)
    Metabolic and bioprocess engineering for productive multistep and redox biocatalysis.
    2nd Biocatalysis Symposium, EMPA, 16. January 2012, EMPA, Dübendorf, Switzerland
2011
  • Bühler B., Collins J., Brandenbusch C., Kuhn D., Park J. B., Schrewe M., Blank L. M., Sadowski G. and Schmid A. (2011)
    Whole-cell biocatalysis in organic-aqueous two-liquid phase systems.
    Frontiers 2011, 21. - 22. June 2011, Delft, The Netherlands
  • Bühler B. (2011)
    Rational strain and process engineering for efficient whole-cell redox biocatalysis.
    DBU-Workshop „Effiziente Biokatalysatoren für die Weiße Biotechnologie“, 2. - 3. May 2011, Osnabrück, Germany
2010
  • Bühler B., Kuhn D., Ebert B. E., Blank L. M. and Schmid A. (2010)
    Ecoefficiency and metabolic evaluation of recombinant microbial biocatalysts for specific carbon oxyfunctionalization.
    BioTrends 2010, 1. - 2. December 2010, Dortmund, Germany
  • Bühler B., Blank L. M. and Schmid A. (2010)
    Biocatalysis meets Systems Biotechnology.
    Jahrestagung der Biotechnologen, 21. - 23. September, Aachen, Germany
  • Bühler B., Falcioni F., Frick O., Fu J., Blank L. M., Bühler K. and Schmid A. (2010)
    Coupling of central carbon metabolism to selective proline hydroxylation by α-ketoglutarate-dependent proline 4-hydroxylase.
    ESBES (European Symposium on Biochemical Engineering Science), 5. - 8. March 2010, Bologna, Italy
2009
  • Bühler B., Bühler K., Blank L. M. and Schmid A. (2009)
    The use of oxidoreductases for biocatalytic oxyfunctionalization.
    NAWI Graz Workshop, 26th of June 2009, Graz, Austria
  • Bühler B. (2009)
    The use of oxidoreductases for biocatalytic oxyfunctionalization.
    GDCh-Vortrag, Greifswald, Germany
2008
  • Bühler B., Kuhn D., Ebert B. E., Kholiq A., Heinzle E., Blank L. M. and Schmid A. (2008)
    Using Systems Biotechnology and Eco-Efficiency Analyses to Enable Sustainable Redox Biocatalysis.
    Bioperspectives 2008, 7th - 9th of October, Hannover, Germany
  • Bühler B., Ionidis G., Park J. B., Ebert B. E., Kuhn D., Blank L. M. and Schmid A. (2008)
    Systems Biotechnology for Selective Whole-Cell Biocatalysis.
    ESBES 2008, 7th - 10th of September 2008, Faro, Portugal
  • Bühler B. (2008)
    Whole-Cell Biocatalysis for Carbon Oxyfunctionalization.
    Seminar at the Medical Proteome Center Bochum, 9th of May 2008, Bochum, Germany
2007
  • Bühler B., Ebert B. E., Blank L. M. and Schmid A. (2007)
    Systems biotechnology of resting Escherichia coli cells as a platform for sustainable redox biocatalysis.
    European BioPerspectives, 30. May - 1. June 2007, Cologne, Germany
2006
  • Bühler B. and Schmid A. (2006)
    Whole-cell biocatalysis for the oxyfunctionalization of hydrocarbons - efficiency and limitations.
    24. DECHEMA-Jahrestagung der Biotechnologen, 26. - 28. September 2006, Wiesbaden, Germany
  • Bühler B. and Schmid A. (2006)
    Whole-cell biocatalysis for the oxyfunctionalization of hydrocarbons-efficiency and limitations.
    Institute of Biotechnology, Forschungszentrum Jülich, Germany
2005
  • Bühler B., Straathof A. J. J. and Schmid A. (2005)
    Whole-cell bio-oxidation in a two-liquid phase system. What are limiting factors?
    Biocatalysis in Nonconventional Media, 13. - 14. April 2005, Manchester, UK
  • Bühler B., Park J. B., Witholt B. and Schmid A. (2005)
    Application of oxygenases in whole-cell biocatalysis; two examples.
    Public Lecture at the Department of Biology, University of Konstanz, Germany
2004
  • Bühler B., Straathof A. J. J. and Schmid A. (2004)
    Modeling and simulation of a whole-cell multistep biooxidation process in a two-liquid phase system.
    5th European Symposium on Biochemical Engineering Science, ESBES-5, 8. - 11. September 2004, Stuttgart, Germany
2001
  • Bühler B., Witholt B., Hauer B. and Schmid A. (2001)
    Multistep biocatalysis with Xylene Monooxygenase containing recombinant E. coli; Production of 3,4-dimethylbenzaldehyde from pseudocumene.
    VAAM-Biotransformation Jahrestagung, Berlin, Germany
Posters
2015
  • Grund M., Frick O., Bühler B. and Schmid A. (2015)
    Towards quantitative physiology in cyanobacteria by isotopically instationary 13C-metabolic flux analysis .
    DECHEMA Summer School Quantitative Biology 2015, 20. - 24. July 2015, Berlin, Germany
  • Grund M., Krabbe P., Frick O., Bühler B. and Schmid A. (2015)
    Towards quantitative physiology in cyanobacteria.
    1st International Solar Fuels Conference (ISF-1), 26. April - 01. May 2015, Uppsala, Sweden
  • Grund M., Frick O., Bühler B. and Schmid A. (2015)
    Metabolic Engineering of Cyanobacteria as Efficient Industrial Biocatalysts.
    UFZ EnergyDays 2015, 24. - 25. February 2015, Leipzig, Germany
2014
  • Ladkau N., Schrewe M., Schmid A. and Bühler B. (2014)
    Three-step sequential whole-cell biocatalysis for the technical scale production of 12-aminododecanoic acid methyl ester from renewables.
    3rd Multistep Enzyme Catalyzed Processes Congress (MECP14), 7. - 10. April 2014, Madrid, Spain
  • Grund M., Frick O., Bühler B. and Schmid A. (2014)
    Metabolic Engineering of Cyanobacteria as Efficient Industrial Biocatalysts.
    DECHEMA Summer School Biotransformations 2014, 24. - 27. August 2014, Bad Herrenalb, Germany
  • David C., Grund M., Bühler B., Bühler K. and Schmid A. (2014)
    Applied Biocatalysis & Bio-Artificial Photosynthesis.
    POF III Evaluation Meeting, 4. February 2014, Berlin, Germany
  • Theodosiou E., Frick O., Bühler B. and Schmid A. (2014)
    Metabolic engineering of E. coli for efficient and high yielding trans-4-hydroxy-L-proline production from L-proline.
    10th European Symposium on Biochemical Engineering Sciences and 6th International Forum on Industrial Bioprocesses, 07.-10. September 2014, Lille, France
  • Theodosiou E., Frick O., Falcioni F., Bühler B. and Schmid A. (2014)
    Metabolic flux analysis unravels the interconnection between cell physiology and biocatalytic activity of E. coli containing recombinant proline-4-hydroxylase.
    Systems Biology Course, 02. - 08. March 2014, Innsbruck, Austria
2013
  • Brandenbusch C., Glonke S., Collins J., Bühler B., Schmid A. and Sadowski G. (2013)
    Stable emulsions in biphasic whole-cell biocatalysis: The mechanism of scCO2-assisted phase separation.
    ProcessNet Jahrestagung der Fachgemeinschaft Fluiddynamik und Trenntechnik, 25. - 27. September 2013, Würzburg, Germany
  • Willrodt C., David C., Julsing M. K., Bühler B. and Schmid A. (2013)
    Biocatalyst and process development for fermentative synthesis of monoterpene-derived natural product.
    1st European Conference on Natural Products, 22. - 23. September 2013, Frankfurt am Main, Germany
  • Willrodt C., David C., Julsing M. K., Bühler B. and Schmid A. (2013)
    Biocatalyst and process development for the fermentative synthesis of monoterpene-derived natural product.
    Biotrans 2013, 21. - 25. July 2013, Manchester, United Kingdom
  • Schrewe M., Ladkau N., Lange K., Czarnotta E, Julsing M. K., Bühler B. and Schmid A. (2013)
    Reaction and catalyst engineering for whole-cell-based multistep ω-functionalization of renewables: Sustainable and efficient production of bifunctional building blocks for polymer syntheses.
    Biotrans 2013, 21. - 25. July 2013, Manchester, UK
  • Ladkau N., Schrewe M., Aßmann M., Klassen G., Bühler B. and Schmid A. (2013)
    Strain engineering for the omega-aminofunctionalization of dodecanoic acid methyl ester via multistep biocatalysis.
    Biotrans 2013, 21. - 25. July 2013, Manchester, UK
  • Lindmeyer M., Schmid A. and Bühler B. (2013)
    Variability analysis of different Pseudomonas strains during oxidative whole‐cell biocatalysis.
    Biotrans 2013, 21. - 25. July 2013, Manchester, UK
  • Falcioni F., Theodosiou E., Frick O., Schmid A. and Bühler B. (2013)
    Whole-cell biocatalysis for the conversion of L-proline to trans-4-hydroxy-L-proline with high specificity, yield, and productivity.
    Biotrans 2013, 21. - 25. July 2013, Manchester, UK
  • Lange K., Julsing M. K., Bühler B. and Schmid A. (2013)
    Biocatalyst design for the production of cannabinoids.
    CLIB Retreat, 13. February 2013, Lünen, Germany
  • Willrodt C., David C., Julsing M. K., Bühler B. and Schmid A. (2013)
    Preparation of monoterpenes from cheap and renewable resources.
    Tag der Chemie, TU Dortmund, 1. February 2013, Dortmund, Germany
2012
  • Theodosiou E., Frick O., Bühler B. and Schmid A. (2012)
    Metabolic engineering of E.coli for the production of trans-4-hydroxy-L-proline.
    Metabolic Engineering IX, 3. - 7. June 2012, Biarritz, France
  • Volmer J., Neumann C., Schmid A. and Bühler B. (2012)
    Engineering Pseudomonas sp. strain VLB120 for redox biocatalysis with toxic reactants (Poster prize!).
    ESBES - 9th European Symposium on Biochemical Engineering Science, 23. - 26. September 2012, Istanbul, Turkey
  • Volmer J., Neumann C., Schmid A. and Bühler B. (2012)
    Towards a stable solvent tolerant phenotype of Pseudomonas sp. strain VLB120 for redox biocatalysis with toxic reactants.
    3rd annual CLIB-graduate cluster retreat, 22. - 24. February 2012, Bergisch-Gladbach, Germany
  • Lindmeyer M., Schmid A. and Bühler B. (2012)
    The catalytic efficiency and expression of oxygenases in Pseudomonas.
    Biotrends 2012, 29. - 30. November 2012, Dortmund, Germany
  • Lindmeyer M., Schmid A. and Bühler B. (2012)
    The catalytic efficiency and expression of oxygenases in Pseudomonas.
    ProcessNet-Jahrestagung 2012, 10. - 13. September 2012, Karlsruhe, Germany
  • Lange K., Hoschek A, Bühler B., Julsing M. K. and Schmid A. (2012)
    Functional expression of the THCA synthase from Cannabis sativa in E. coli.
    Biocat 2012, 31. August - 4. September 2012, Hamburg, Germany
  • Willrodt C., Julsing M. K., Bühler B. and Schmid A. (2012)
    Monoterpenoid production in metabolically engineered Escherichia coli.
    Biocat 2012, 31. August - 4. September 2012, Hamburg, Germany
  • Ladkau N., Schrewe M., Julsing M. K., Bühler B. and Schmid A. (2012)
    Specific terminal oxy- and aminofunctionalization of dodecanoic acid methyl ester coupled in a single whole-cell catalyst.
    Gordon Research Conference Biocatalysis, 8. - 13. July 2012, Smithfield, RI, USA
  • Julsing M. K., Schrewe M., Cornelissen S., Schmid A. and Bühler B. (2012)
    The outer membrane protein AlkL: a tool for the microbial biotransformation of hydrophobic substrates.
    Gordon Research Conference Biocatalysis, 8. - 13. July 2012, Smithfield, RI, USA
  • Rensmann L., Maaß M., Teubner N.C., Bühler B., Julsing M. K. and Schmid A. (2012)
    Enzyme titer and specific activity of recombinant human CYP450 1A1 and 2D6 in function of cell physiology in a whole-cell set-up.
    11th International symposium on cytochrome P450. Biodiversity and Biotechnology., 22. - 26. June 2012, Turin, Italy, http://p450-torino.it/
  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2012)
    Resting cells of recombinant E. coli show high epoxidation yields on energy source and high sensitivity to product inhibition.
    Catalyzing Bio-Economy - Biocatalysts for Industrial Biotechnology, Annual meeting of the DECHEMA-VAAM-Section Biotransformations, 24. - 25. April 2012, Frankfurt am Main, Germany
  • Schmutzler K., Volmer J., Halan B., Bühler B., Bühler K. and Schmid A. (2012)
    Solvent tolerance in Pseudomonas sp. strain VLB120 - biofilms vs planktonic cells.
    VAAM Jahrestreffen, 18. - 21. March 2012, Tübingen, Germany
  • Lange K., Julsing M. K., Bühler B. and Schmid A. (2012)
    Functional expression of the THCA synthase from Cannabis sativa in E. coli.
    CLIB Retreat, 22. - 24. February 2012, Bergisch Galdbach, Germany
2011
  • Volmer J., Schmid A. and Bühler B. (2011)
    Solvent tolerant phenotypes of Pseudomonas sp. strain VLB120 - a chance to improve redox biocatalysis with toxic reactants?
    2nd Annual CLIB-Graduate cluster retreat, 7. April 2011, Bonn, Germany
  • Ladkau N., Hermann I., Bühler B. and Schmid A. (2011)
    Enzyme catalyzed laurolactam synthesis in aqueous solution.
    BioTrends 2011, 8.-9. December 2011, Dortmund, Germany
  • Schrewe M., Ladkau N., Lange K., Czarnotta E, Julsing M. K., Bühler B. and Schmid A. (2011)
    Multi-step whole-cell biocatalysis for the specific functionalization of unactivated terminal C‑H bonds.
    BioTrends 2011, 8. – 9. December 2011, Dortmund, Germany
  • Collins J., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2011)
    Dynamic cell based emulsions in biphasic biocatalytic systems and strategies for their downstream processing.
    Biotrans 2011, 2. - 6. October 2011, Giandini Naxos, Sicily, Italy
  • Ladkau N., Hermann I., Bühler B. and Schmid A. (2011)
    Enzyme catalyzed laurolactam synthesis via intramolecular amide-bond formation in aqueous solution.
    Biotrans 2011, 2.-6. October 2011, Giardini Naxos, Sicily, Italy
  • Julsing M. K., Schrewe M., Cornelissen S., Schmid A. and Bühler B. (2011)
    Improved biotransformation rates for hydrophobic substrates using ths outer membrane protein AlkL in E. coli.
    Biotrans 2011, 2. - 6. October 2011, Giardini Naxos, Sicily, Italy
  • Falcioni F., Frick O., Blank L. M., Schmid A. and Bühler B. (2011)
    Relationship between cell physiology and biocatalytic activity in E. coli expressing proline-4-hydroxylase.
    Biotrans 2011, 2. - 6. October 2011, Giardini Naxos, Sicily, Italy
  • Schrewe M., Ladkau N., Lange K., Czarnotta E, Julsing M. K., Bühler B. and Schmid A. (2011)
    Whole-cell biocatalysis for the controlled terminal functionalization of sp3 hybridized C‑H bonds.
    Biotrans 2011, 2. - 6. October 2011, Giardini Naxos, Sicily, Italy
  • Lange K., Cornelissen S., Julsing M. K., Bühler B. and Schmid A. (2011)
    Biocatalyst design for the synthesis of cannabinoids in a microbial host: metabolic engineering for the production of monoterpenoids.
    1st European Congress of Applied Biotechnology, 25. - 29. September 2011, Berlin, Germany
  • Collins J., Brandenbusch C., Sadowski G., Schmid A. and Bühler B. (2011)
    Dynamic cell based emulsions in biphasic biocatalytic systems and strategies for their downstream processing.
    1st European Congress of Applied Biotechnology, 25. - 29. September 2011, Berlin, Germany
  • Hillen M, Frick O., Falcioni F., Schmid A. and Bühler B. (2011)
    Metabolic analysis and optimization of a recombinant Escherichia coli as a biocatalyst for the production of trans-4-hydroxy-L-proline from L-proline.
    2ND SUMMER SCHOOL Biotransformations, 22. - 25. August 2011, Bad Herrenalb, Germany
  • Lange K., Cornelissen S., Julsing M. K., Bühler B. and Schmid A. (2011)
    Production of cannabinoids in a microbial host: synthesis of THCA in E. coli.
    CLIB Retreat, 7. April 2011, Bonn, Germany
  • Lang K., Bühler B. and Schmid A. (2011)
    Development of an isobutanol producing biofilm catalyst.
    2nd CLIB international conference, 5. April 2011, Düsseldorf, Germany
  • Volmer J., Schmid A. and Bühler B. (2011)
    Solvent tolerant phenotypes of Pseudomonas sp. strain VLB120 - a chance to improve redox biocatalysis with toxic reactants?
    CLIB Conference, 5. - 6. April 2011, Düsseldorf, Germany
  • Fu J., Falcioni F., Bühler B., Bühler K. and Schmid A. (2011)
    Biochemical characterization of P4Hs: Towards hydroxyproline production by recombinant E. coli..
    DECHEMA Summerschool , September 2011, Bad Herrenalb, Germany
  • Volmer J., Neumann C., Schmid A. and Bühler B. (2011)
    Towards a stable solvent tolerant phenotype for redox biocatalysis with Pseudomonas sp. strain VLB120 .
    1st European Congress of Applied Biotechnology, 25. – 29. September 2011, Berlin, Germany
2010
  • Volmer J., Bühler B. and Schmid A. (2010)
    Pseudomonas strains - a platform for redox biocatalysis.
    1st Annual CLIB-Graduate cluster retreat, 8. March 2010, Wuppertal, Germany
  • Ütkür F.Ö., Tran T.-T., Schmid A. and Bühler B. (2010)
    Development of a two-liquid phase process for oxyfunctionalizations catalyzed by dehyrogenase-containing microbial cells.
    ESBES (European Symposium on Biochemical Engineering Science), 5. - 8. September 2010, Bologna, Italy
  • Kuhn D., Zhang X., Fritzsch F. S. O., Blank L. M., Schmid A. and Bühler B. (2010)
    Systems biotechnological approach for intensification of a biocatalytic oxyfunctionalization process.
    ESBES (European Symposium on Biochemical Engineering Science), 5. - 8. September 2010, Bologna, Italy
  • Volmer J., Schmid A. and Bühler B. (2010)
    Solvent tolerant Pseudomonas strains - a platform for Redox Biocatalysis with Toxic Reactants.
    ESF-Bielefeld-CeBiTec Conference; Microbes and Industrial Biotechnology, 21.-24. November 2010, Bielefeld, Germany
  • Ütkür F.Ö., Tran T.-T., Schmid A. and Bühler B. (2010)
    Development of a Two-Liquid Phase Process for Oxyfunctionalizations Catalyzed by Dehydrogenase-Containing Microbial Cells.
    BioTrends 2010, 1. - 2. December 2010, Dortmund, Germany
  • Falcioni F., Frick O., Blank L. M., Schmid A. and Bühler B. (2010)
    Microbial physiology determines the catalytic efficiency of proline hydroxylation by proline-4-hydroxylase.
    BioTrends 2010, 1. - 2. December 2010, Dortmund, Germany
  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2010)
    Oxygenase catalyzed styrene epoxidation using non-growing E.coli cells in a two-liquid phase system.
    BioTrends 2010, 1. - 2. December 2010, Dortmund, Germany
  • Volmer J., Schmid A. and Bühler B. (2010)
    Solvent tolerant Pseudomonas strains - a platform for Redox Biocatalysis with Toxic Reactants.
    BioTrends, 1. - 2. December 2010, Dortmund, Germany
  • Ladkau N., Schrewe M., Bühler B. and Schmid A. (2010)
    Multistep-Biocatalysis for the production of 12-aminolauric acid methyl ester from lauric acid methyl ester with recombinant E. coli.
    EFB Bioprocess Engineering Course, 19. - 26. September 2010, Supetar, Croatia
  • Escapa I. F., Nogales J., Díaz E., Bühler B., Blank L. M. and Prieto M.A. (2010)
    Influence of polyhydroxyalkanoate content on central carbon metabolism operation of Pseudomonas putida KT2442.
    Systems Biology of Microorganisms, 22. - 24. March 2010, Paris, France
  • Bühler B., Cornelissen S. and Schmid A. (2010)
    Cell physiology determines the efficiency of cytochrome P450-catalyzed C-H-oxyfunctionalization.
    Gordon Research Conference on Biocatalysis, 11. - 16. July 2010, Smithfield, RI, USA
  • Falcioni F., Frick O., Blank L. M., Schmid A. and Bühler B. (2010)
    Interrelation between α-ketoglutarate dependent proline hydroxylation and metabolic network operation in recombinant E. coli.
    Gordon Research Conference on Biocatalysis, 11. - 16. July 2010, Smithfield, RI, USA
2009
  • Schrewe M., Schmid A. and Bühler B. (2009)
    Application of a whole-cell biocatalyst for the omega-oxyfunctionalizationof fatty acid methyl ester.
    Biotrans 2009, 5. – 9. July 2009, Bern, Switzerland
  • Falcioni F., Kleinsteuber P., Bühler B. and Schmid A. (2009)
    How to improve proline hydroxylation in a whole-cell biocatalyst.
    Biotrans 2009, 5. – 9. July 2009, Bern, Switzerland
  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2009)
    The potential of resting E. coli cells for oxygenase catalysis.
    Biotrans 2009, 5. – 9. July 2009, Bern, Switzerland
  • Bühler B., Cornelissen S., Liu S and Schmid A. (2009)
    Whole-cell biocatalysis: An efficient strategy for specific C-H oxyfunctionalizations - P450 catalysis.
    Biotrans 2009, 5. - 9. July 2009, Bern, Switzerland
  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2009)
    A comparison of resting and growing E. coli for oxygenase catalysis.
    DECHEMA Jahrestagung der Biotechnologen, 8. - 10. September 2009, Mannheim, Germany
  • Ütkür F.Ö., Sulu D. K., Schmid A. and Bühler B. (2009)
    Quinoline 2-oxidoreductase (Qor): A promising candidate for carbon oxyfunctionalizations.
    Biotrans 2009, 5. - 9. July 2009, Bern, Switzerland
  • Cornelissen S., Liu S, Bühler B. and Schmid A. (2009)
    Substrate availability limits CYP153A6-catalyzed (S)-perillyl alcohol production in two-liquid phase biotransformations.
    16th International Conference on Cytochrome P450, Biochemistry, Biophysics, Biotechnology, 21. - 25. June 2009, Nago (Okinawa), Japan
2008
  • Brandenbusch C., Bühler B., Schmid A. and Sadowski G. (2008)
    Innovative down-stream processing in whole-cell biocatalysis using supercritical carbon dioxide.
    2. International Symposium on Biothermodynamics, Dechema, 21.- 22. February 2008, Frankfurt, Germany
  • Bayraktar F. Ö., Gaykawad S., Schmid A. and Bühler B. (2008)
    Application of quinaldine 4-oxidase-containing recombinant P. putida as a biocatalyst in hydroxylation reactions.
    ESBES 2008, 7. - 10. September 2008, Faro, Portugal
  • Schrewe M., Bühler B. and Schmid A. (2008)
    Development of a productive whole-cell biocatalyst for ω-hydroxylation of fatty acid methyl esters.
    Biocat 2008, 31. August - 4. September 2008, Hamburg, Germany
  • Falcioni F., Kleinsteuber P., Bühler B. and Schmid A. (2008)
    Microbial proline-hydroxylase: a model for α-ketoglutarate dependent oxygenases.
    Biocat 2008, 31. August - 4. September 2008, Hamburg, Germany
  • Bühler B., Bayraktar F. Ö., Gaykawad S. and Schmid A. (2008)
    Application of molybdenum-containing dehydrogenases for carbon oxyfunctionalization.
    Gordon Conference on Biocatalysis 2008, 6. - 11. July 2008, Smithfield, RI, USA
  • Brandenbusch C., Bühler B., Schmid A. and Sadowski G. (2008)
    Innovative down-stream processing in whole-cell biocatalysis using supercritical carbon dioxide.
    2nd International Symposium on Biothermodynamics, 21. - 22. February 2008, Frankfurt/Main, Germany
2007
  • Cornelissen S., Julsing M. K., Bühler B. and Schmid A. (2007)
    Evaluation of different recombinant microorganisms expressing mammalian CYP1A1 for productive whole-cell biocatalysis.
    15th International Conference on Cytochromes P450, Biophysics and Functional Genomics, 17. - 21. July 2007, Bled, Slovenia
  • Bayraktar F. Ö., Bühler B. and Schmid A. (2007)
    Application of molybdenum-containing hydroxylases in hydroxylation reactions.
    Biotrans 2007 - 8th International Symposium on Biocatalysis and Biotransformations, 8. - 13. July 2007, Ovideo, Spain
  • Bühler B., Kuhn D., Park J. B., Blank L. M. and Schmid A. (2007)
    Evaluation of the efficiency of recombinant Escherichia coli as an epoxidation catalysts by a systems biotechnology approach.
    Biotrans 2007 - 8th International Symposium on Biocatalysis and Biotransformations, 8. - 13. July 2007, Ovideo, Spain
  • Blank L. M., Ionidis G., Bühler B. and Schmid A. (2007)
    Estimation of the microbial redox cofactor regeneration rate.
    European BioPerspectives, 30. May - 1. June 2007, Cologne, Germany
  • Ebert B. E., Blank L. M., Bühler B. and Schmid A. (2007)
    Metabolic capacity estimation of Escherichia coli as platform for redox biocatalysis.
    European BioPerspectives, 30. May - 1. June 2007, Cologne, Germany
  • Ebert B. E., Blank L. M., Bühler B. and Schmid A. (2007)
    Using constraint-based modeling to estimate Escherichia colis capacity for redox biocatalysis.
    2nd FEBS Advanced Lecture Course on Systems Biology, 11. - 16. March 2007, Gosau, Austria
  • Kuhn D., Kholiq A., Heinzle E., Bühler B. and Schmid A. (2007)
    Sustainability evaluation and optimization of a biocatalytic two-liquid phase epoxidation process.
    Biotrans 2007 - 8th International Symposium on Biocatalysis and Biotransformations, 8. - 13. July 2007, Oviedo, Spain
2006
  • Blank L. M., Ebert B. E., Bühler B. and Schmid A. (2006)
    Metabolic capacitiy of biocatalytic active microbes.
    6th Metabolic Engineering Conference, 1. - 5. October 2006, Nordwijkerhout, Netherlands
  • Blank L. M., Ebert B. E., Bühler B. and Schmid A. (2006)
    Constrained based modelling for metabolic capacity estimation of Escherichia coli as platform for redox biocatalysis .
    From µl to m3 - Strategies in bioprocess development, 28. - 29. March 2006, Hamburg, Germany
  • Bühler B., Park J. B. and Schmid A. (2006)
    Recombinant whole-cell biocatalysis for highly selective epoxidation - a comparison of resting and growing cells.
    From µl to m3 - Strategies in bioprocess development, 28. - 29. March 2006, Hamburg, Germany
  • Blank L. M., Ionidis G., Bühler B. and Schmid A. (2006)
    Quantitative physiology of microbes; metabolic capacitiy of Pseudomonas putida for biocatalysis.
    20th International Symposium on Micro-Scale Bioseparations, 21. - 25. January 2006, Amsterdam, Netherlands
2004
  • Bühler B., Straathof A. J. J., Witholt B. and Schmid A. (2004)
    Modeling and simulation of a whole-cell multistep biooxidation process in a two liquid phase system.
    5th D-BIOL Symposium, 19. - 21. May 2004, Davos, Switzerland
2003
  • Bühler B., Straathof A. J. J., Witholt B. and Schmid A. (2003)
    Modeling and simulation of a whole-cell multistep biooxidation process in a two liquid phase system.
    11th European Congress on Biotechnology, 24. - 29. August 2003, Basel, Switzerland
2002
  • Bühler B., Bollhalder I., Feiten H.-J., Hauer B., Witholt B. and Schmid A. (2002)
    Pilot-scale production of 3,4-Dimethylbenzaldehyde from pseudocumene via multistep biocatalysis in a Xylene Monooxygenase based two-liquid phase process.
    4th D-BIOL Symposium, 8. - 10. May 2002, Davos, Switzerland


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