People
Dr. Eleni Theodosiou
Department of Solar Materials
Helmholtz Centre for Environmental Research
Kubus, Room 412
Permoserstraße 15
04318 Leipzig
Tel.: +49-341-235 46 91
Fax.: +49-341-235 45 12 86
E-Mail:
Resume
Theodosiou Eleni studied Food Science and Technology from 1996 to 2001 at the Aristotle University of Thessaloniki (AUTH) with specialization on Food Biotechnology and Microbiology. During her master thesis (2001-2003) she worked at the Laboratory of Food Engineering and Processing (AUTH) on the optimization of β-carotene production by Blakeslea trispora. In her Ph.D. (2004-2009) in the group of Prof. Kolisis, at the Department of Chemical Engineering (National Technical University of Athens), she investigated the biotransformation of natural compounds using hydrolases in non-conventional media. During her Ph.D. (2004-2006) she cooperated with the Department of Conservation of Antiquities and Works of Art (TEI of Athens) for the development of enzymatic methods for the cleaning of museum objects. From 2007 to 2008 she worked as guest researcher in the group of Pro. Bornscheuer (Dept. of Biotechnology & Enzyme Catalysis, University of Greifswald) where she gained hands-on experience on strategies for the design of tailor made biocatalysts.
Research Interests
- Applied biocatalysis and biotransformations
- Industrial Microbiology
- Metabolic engineering
CV / Scientific Career
| since 01/2015 | Post-doctoral researcher at the Dept. of Solar Materials, Helmholtz Center for Environmental Research - UFZ, Leipzig | ||
| 06/2011-12/2014 | Post-doctoral researcher at the Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund | ||
| 01/2011-05/2011 | Post-doctoral researcher at the Dept. of Molecular Enzyme Technology and Biochemistry, Biofilm Centre, University of Duisburg-Essen, Essen | ||
| 09/2004-09/2009 | PhD at the Biotechnology Laboratory, Dept. of Synthesis and Development of Industrial Processes, School of Chemical Engineering, National Technical University of Athens | ||
| 09/2007-09/2008 | Guest Researcher at the Institute of Biochemistry, Dept. of Biotechnology and Enzyme Catalysis, Ernst-Moritz-Arndt University of Greifswald | ||
| 11/2001-11/2003 | M.Sc. at the Department of Food Science and Technology, Faculty of Agriculture, Aristotle University of Thessaloniki | ||
| 09/1996-11/2001 | B.Sc. Ag. (Hons) at the Department of Food Science and Technology, Faculty of Agriculture, Aristotle University of Thessaloniki |
Grants & Awards
- Postgraduate Scholarships by the Greek State Scholarships Foundation (IKY) (2002-2003, 2005-2007) after nationwide examinations on Food Biotechnology and Microbiology
Publications
Peer Reviewed Articles
2017
- Theodosiou E., Breisch M., Julsing M. K., Falcioni F., Bühler B. and Schmid A. (2017)
An artificial TCA cycle selects for efficient α-ketoglutarate dependent hydroxylase catalysis in engineered Escherichia coli.
Biotechnology and Bioengineering, 114(7):1511-1520, DOI-Link
2015
- Theodosiou E., Frick O., Bühler B. and Schmid A. (2015)
Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation.
Microbial Cell Factories, 14:108, DOI-Link
2013
- Theodosiou E., Purchartová K., Stamatis H., Kolisis F. and Křen V. (2013)
Bioavailability of silymarin: Drug formulations and biotransformation.
Phytochemistry Reviews, , DOI-Link
2011
- Theodosiou E., Loutrari H., Stamatis H., Roussos C. and Kolisis F. (2011)
Biocatalytic synthesis and antitumor activities of novel silybin acylated derivatives with dicarboxylic acids.
New Biotechnology, 28(342-348), DOI-Link
2010
- Xanthakis E., Theodosiou E., Stamatis H., Magkouta S., Loutrari H., Roussos C. and Kolisis F. (2010)
Enzymatic transformation of flavonoids and terpenoids: Structural and functional diversity of the novel derivatives.
Pure and Applied Chemistry, 82(1-16), DOI-Link
2009
- Theodosiou E., Katsoura M. H., Loutrari H., Purchartová K., Křen V., Kolisis F. and Stamatis H. (2009)
Enzymatic preparation of acylated derivatives of silybin in organic and ionic liquids media and evaluation of their anti-tumor proliferative activity.
Biocatalysis and Biotransformations, 27(161-169), DOI-Link
Books & Bookchapters
2009
- Theodosiou E. (2009)
PhD Thesis: Strategies for the biocatalytic modification of natural antioxidants.
National Technical University of Athens, Athens, Greece
- Katsoura M. H., Theodosiou E., Stamatis H. and Kolisis F. (2009)
Strategies for the biocatalytic lipophilization of phenolic antioxidants.
in Fessner W.-D. and Anthonsen T., Modern Biocatalysis: Stereoselective and Environmentally-Friendly Reactions.
Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, DOI-Link
Patent applications
2004
- Roukas T., Mantzouridou F., Theodosiou E., Kotzekidou P. and Liakopoulou-Kiriakidou M. (2004)
Method for producing β-carotene.
OVI 1004614/25-6-04
Oral Presentations
2007
- Theodosiou E., Rapti S., Malea A., Pournou A., Karantoni E., Panagiaris F. and Kolisis F. (2007)
Hydrolytic enzymes and their ability to remove drying oils and resins from cotton and leather made samples.
29th Scientific Conference of the Hellenic Association of Biological Research, 17. - 19. May 2007, Kavala, Greece
- Theodosiou E., Katsoura M. H., Stamatis H. and Kolisis F. (2007)
Biocatalytic synthesis of lipophilic derivatives of a natural antioxidant silybin in ionic liquids and organic solvents: A comparative study.
2nd Panhellenic Congress: Biotechnology and Food Technology, 29. - 31. March 2007, Athens, Greece
Posters
2014
- Theodosiou E., Frick O., Bühler B. and Schmid A. (2014)
Metabolic engineering of E. coli for efficient and high yielding trans-4-hydroxy-L-proline production from L-proline.
10th European Symposium on Biochemical Engineering Sciences and 6th International Forum on Industrial Bioprocesses, 07.-10. September 2014, Lille, France
- Theodosiou E., Frick O., Falcioni F., Bühler B. and Schmid A. (2014)
Metabolic flux analysis unravels the interconnection between cell physiology and biocatalytic activity of E. coli containing recombinant proline-4-hydroxylase.
Systems Biology Course, 02. - 08. March 2014, Innsbruck, Austria
2013
- Falcioni F., Theodosiou E., Frick O., Schmid A. and Bühler B. (2013)
Whole-cell biocatalysis for the conversion of L-proline to trans-4-hydroxy-L-proline with high specificity, yield, and productivity.
Biotrans 2013, 21. - 25. July 2013, Manchester, UK
2012
- Theodosiou E., Frick O., Bühler B. and Schmid A. (2012)
Metabolic engineering of E.coli for the production of trans-4-hydroxy-L-proline.
Metabolic Engineering IX, 3. - 7. June 2012, Biarritz, France
2007
- Theodosiou E., Loutrari H., Magkouta S., Roussos C., Stamatis H. and Kolisis F. (2007)
Dicarboxylic acids as acyl donors for the biocatalytic synthesis of lipophilic derivatives of natural antioxidants: Anti-tumor potential of the novel analogues studied on K562 human leukaemia cells.
5th Euro Fed Lipid Congress, Oils, Fats and Lipids: From Science to Applications, Innovations for a better World, 16. - 19. September 2007, Gothenburg, Sweden
- Theodosiou E., Loutrari H., Magkouta S., Roussos C., Stamatis H. and Kolisis F. (2007)
Acylation of flavonoids with dicarboxylic acids. The potential anti-tumor and anti-angiogenic properties of the novel analogues.
International Conference "Biocatalysis 2007 Structure, functions, applications" , 17. - 22. June 2007, Moscow, St.-Petersburg, Russia
- Theodosiou E., Stamatis H. and Kolisis F. (2007)
New analogues of the natural antioxidant silybin through biocatalytic synthesis: Increase of lipophilicity and synthesis of hybridic antioxidants.
2nd Greek Lipid Forum, National Hellenic Research Foundation, 07. - 08. June 2007, Athens, Greece, Link
2006
- Theodosiou E., Stamatis H. and Kolisis F. (2006)
Lipase catalyzed esterification of silybin and rutin with dicarboxylic acids in media with low water content.
Protein Design and Evolution for Biocatalysis, 30. August - 01. September 2006, Ernst-Moritz-Arndt University, Greifswald, Germany
2005
- Theodosiou E., Stamatis H. and Kolisis F. (2005)
Lipase-catalyzed esterification of rutin with dicarboxylic acids, 57th Hellenic Society for Biochemistry and Molecular Biology Conference, Biomedical Research Foundation, Academy of Athens, Athens, Greece, Dec 9-11, 2005.
57th Hellenic Society for Biochemistry and Molecular Biology Conference, Biome, 09. - 11. December 2005, Athens, Greece
- Theodosiou E., Stamatis H. and Kolisis F. (2005)
Enzymatic modification of rutin with dicarboxylic acids as acyl donors.
1st Greek Lipid Forum, Euro Fed Lipid, National Hellenic Research Foundation, 08. - 09. June 2005, Athens, Greece